| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis)
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| Autores: |
Paulin,Lília Márcia Silva
Samartino,Luis Ernesto
Conde,Sandra Beatriz
Federsoni,Igor Stefan Poppovic
Ferreira,Fernando
Amaku,Marcos
Ferreira Neto,José Soares
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| Data: |
2012-09-01
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| Ano: |
2012
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| Palavras-chave: |
Serological tests
Serodiagnosis
Buffaloes
Brucellosis
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| Resumo: |
The objective of the present study was to compare the performance of three serological tests for diagnosis of Brucella abortus infections in buffaloes (Bubalus bubalis). Serum samples collected from 696 adult females were submitted to the competitive enzyme-linked immunosorbent assay (ELISA-C), (I-ELISA), fluorescence polarization test (FPA), 2-mercaptoethanol test (2-ME) and complement fixation test (CFT). The gold standard was the combination of CFT and 2-ME, considering as positive the reactors in both CFT and 2-ME, and as negative those non-reactors. ROC analyses were done for C-ELISA, I-ELISA and FPA and the Kappa agreement index were also calculated. The best combinations of relative sensitivity (SEr) and relative specificity (SPr) and Kappa were given by C-ELISA (96.9%, 99.1%, and 0.932, respectively) and FPA (92.2%, 97.6 and 0.836, respectively). The C-ELISA and FPA were the most promising confirmatory tests for the serological diagnosis of brucellosis in buffaloes, and for these tests, cut-off values for buffaloes may be the same as those used for bovines.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000900017
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/S0103-84782012005000070
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.42 n.9 2012
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| Direitos: |
info:eu-repo/semantics/openAccess
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